Surface plasmon resonance method

Principle:

This sensitive technique provides the information of the binding interactions between an injected analyte and an immobilized biomolecule in real time without the presence of a non-invasive, label means. As the analyte binds to the immobilized particle, this forms an associated increase in mass that proportionally increases in refractive index which is observed as a shift in the resonance angle. A flow injection analysis configuration is commonly employed in which the analyte of interest, solvated in a buffer solution, is transported across the sensing surface, where it interacts with the immobilized biomolecule. When combined with appropriate surface chemistry, microfluidics and software, this technique can be used in many applications including:

• Affinity analysis
• Kinetic analysis
• Concentration assays
• Active concentration assays
• Binding stoichiometry
• Thermodynamic analysis
• Study of interaction mechanisms
• Dependence of interaction on environmental conditions
• Routine screening
• Ligand-fishing
• Epitope mapping