Wash solution: is used to wash the unbound materials
Stop solution: as for the second antibody that coupled with the enzyme-labelled conjugation. This enzyme will be bound with reagent ( normally added in the end) to cause color development. The color is directly proportional to the amount of bound antibody-antigen interactions. Thereby, the stop solution is used to stop the enzyme-substrate reaction and allows for color to develop.
No comments:
Post a Comment