Wednesday, August 22, 2012

Simple idea for extraction nucleic acids from cell

Principle methods for extraction of nucleic acids from cell

Cell is cultured and harvested
Lyse cell will detergent to disrupt membrane and inactivate nuclease
Using phenol to seperate nucleic acids from proteins ( Note: proteins will be precipitated while nucleic acids remain in aqueous phase)

Separation of DNA from RNA is based on its solubility in alcoohol
Using DNase or RNase to remove the nucleic acids that is not necessary

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